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Liu Wen, Li Fen, Wang Ping, Liu Xin, He Jing-Jing, Xian Mei-Lin, Zhao Li, Qin Wen, Gan Ren-You, Wu Ding-Tao. Effects of drying methods on the physicochemical characteristics and bioactivities of polyphenolic-protein-polysaccharide conjugates from Hovenia dulcis.
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发布时间：2020-05-04
作者：甘人友（通讯作者）
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| 作者: Liu, W (Liu, Wen); Li, F (Li, Fen); Wang, P (Wang, Ping); Liu, X (Liu, Xin); He, JJ (He, Jing-Jing); Xian, ML (Xian, Mei-Lin); Zhao, L (Zhao, Li); Qin, W (Qin, Wen); Gan, RY (Gan, Ren-You); Wu, DT (Wu, Ding-Tao) | ||||||||||
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| 影响因子 (2018 年): 4.784 | ||||||||||
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| 摘要: Four drying methods, including freeze drying, hot air drying, vacuum drying, microwave drying at 400, 600, and 800 W, were applied to dry the peduncles of Hovenia dulcis. Then the effects of different drying methods on physicochemical characteristics and bioactivities of polyphenolic-protein-polysaccharides conjugates extracted from H. dulcis (PPPs) were investigated and compared. Results showed that different drying methods affected the physicochemical characteristics and bioactivities of PPPs. Noticeable variations in extraction yields, contents of total proteins, contents of total polyphenolics, contents of total flavonoids, contents of uronic acids, molecular weights, apparent viscosities, molar ratios of constituent monosaccharides, ratios of constituent amino acids, and degrees of esterification were observed in PPPs obtained by different drying methods. Besides, a total of 13 phenolic compounds in PPPs were identified by UPLC-ESI-QTOF-MS. In addition, PPPs, especially PPP-M6 and PPP-V dried by microwave drying at 600 W and vacuum drying, respectively, exhibited remarkable antioxidant activities, antiglycation activities, and inhibitory activities on a-amylase and a-glucosidase. Results suggested that the microwave drying and vacuum drying techniques could be appropriate drying methods before extraction of PPPs with high bioactivities for applications in the functional food and medicine industries. (C) 2019 Elsevier B.V. All rights reserved. | ||||||||||
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| 语言: English | ||||||||||
| 文献类型: Article | ||||||||||
| 作者关键词: Hovenia dulcis; Polyphenolic-protein-polysaccharide; Physicochemical characteristic; Antioxidant activity; Antiglycation activity; Enzyme inhibition | ||||||||||
| KeyWords Plus: ANTIOXIDANT ACTIVITY; STRUCTURAL-CHARACTERIZATION; INHIBITORY-ACTIVITY; OFFICINALIS L.; ALPHA-AMYLASE; PEDUNCLES; EXTRACTION; QUALITY; THUNB.; PHYTOCHEMICALS | ||||||||||
| 地址: [Liu, Wen; Li, Fen; Wang, Ping; Liu, Xin; He, Jing-Jing; Xian, Mei-Lin; Zhao, Li; Qin, Wen; Wu, Ding-Tao] Sichuan Agr Univ, Coll Food Sci, Inst Food Proc & Safety, Yaan 625014, Sichuan, Peoples R China. [Gan, Ren-You] Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China. |
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| 通讯作者地址: Wu, DT (通讯作者)，Sichuan Agr Univ, Coll Food Sci, Inst Food Proc & Safety, Yaan 625014, Sichuan, Peoples R China. Gan, RY (通讯作者)，Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China. |
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| 电子邮件地址: ganrenyou@caas.cn; DT_Wu@sicau.edu.cn | ||||||||||
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| Web of Science 类别: Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science | ||||||||||
| 研究方向: Biochemistry & Molecular Biology; Chemistry; Polymer Science | ||||||||||
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This work was supported by the Scientific Research Foundation of Sichuan Agricultural University (grant number 03120321), the Scientific Research Fund Project of Department of Science and Technology of Sichuan Province (grant number 2018JY0149), the National Natural Science Foundation of China (grant number 31901690), and the Technology Innovation Program of Chinese Academy of Agricultural Sciences (grant number ASTIP). |