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Fu Yuan, Li Fen, Ding Ye, Li Hua-Yu, Xiang Xian-Rong, Ye Qiong, Zhang Jian, Zhao Li, Qin Wen, Gan Ren-You, Wu Ding-Tao. Polysaccharides from loquat (Eriobotrya japonica) leaves: Impacts of extraction methods on their physicochemical characteristics and biological activities.
来源:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发布时间:2020-05-02
作者:甘人友(通讯作者)
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作者: Fu, Y (Fu, Yuan); Li, F (Li, Fen); Ding, Y (Ding, Ye); Li, HY (Li, Hua-Yu); Xiang, XR (Xiang, Xian-Rong); Ye, Q (Ye, Qiong); Zhang, J (Zhang, Jian); Zhao, L (Zhao, Li); Qin, W (Qin, Wen); Gan, RY (Gan, Ren-You); Wu, DT (Wu, Ding-Tao) | ||||||||||
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影响因子 (2018 年): 4.784 | ||||||||||
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摘要: Impacts of hot water extraction (HWE), pressurized water extraction (PWE), high-speed shearing homogenization extraction, microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), ultrasound assisted enzymatic extraction, and ultrasound-microwave assisted extraction (UMAE) on physicochemical characteristics and bioactivities of polysaccharides from loquat (Eriobotrya japonica) leaves (LLPs) were investigated. Results showed that the degrees of esterification, contents of phenolics and uronic acids, constituent monosaccharides, apparent viscosities, and molecular weights of LLPs varied by different extraction methods. Bioactivities of LLPs were also significantly affected by different extraction methods. The high molecular weight and high degree of esterification of LLP-W and LIP-P extracted by HWE and PWE, respectively, might contribute to their strong binding capacities. The strong antioxidant activities and inhibitory effects on a-amylase and a-glucosidase were found in LLP-M and LIP-U extracted by MAE and UAE, respectively, which might be attributed to their contents of uronic adds, contents of total phenolics, and molecular weights. The low molecular weights and viscosities of LLP-U and LLP-UM extracted by UMAE might contribute to their strong prebiotic effects. These findings could provide scientific foundations for selecting appropriate extraction methods to obtain LLPs with desired bioactivities for applications in the pharmaceutical and functional food industries. (C) 2020 Elsevier B.V. All rights reserved. | ||||||||||
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语言: English | ||||||||||
文献类型: Article | ||||||||||
作者关键词: Loquat; Extraction method; Polysaccharide; Chemical structure; Bioactivity | ||||||||||
KeyWords Plus: ASSISTED ENZYMATIC EXTRACTION; WATER-SOLUBLE POLYSACCHARIDES; SPEED SHEARING HOMOGENIZATION; DIFFERENT MOLECULAR-WEIGHT; MOMORDICA-CHARANTIA L.; STRUCTURAL-CHARACTERIZATION; ANTIOXIDANT ACTIVITY; GREEN TEA; OPTIMIZATION; PURIFICATION | ||||||||||
地址: [Fu, Yuan; Li, Fen; Ding, Ye; Li, Hua-Yu; Xiang, Xian-Rong; Ye, Qiong; Zhang, Jian; Zhao, Li; Qin, Wen; Wu, Ding-Tao] Sichuan Agr Univ, Coll Food Sci, Inst Food Proc & Safety, Yaan 625014, Sichuan, Peoples R China. [Gan, Ren-You] Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China. |
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通讯作者地址: Wu, DT (通讯作者),Sichuan Agr Univ, Coll Food Sci, Inst Food Proc & Safety, Yaan 625014, Sichuan, Peoples R China. Gan, RY (通讯作者),Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China. |
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电子邮件地址: ganrenyou@caas.cn; DT_Wu@sicau.edu.cn | ||||||||||
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Web of Science 类别: Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science | ||||||||||
研究方向: Biochemistry & Molecular Biology; Chemistry; Polymer Science | ||||||||||
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This work was supported by the Scientific Research Foundation of Sichuan Agricultural University (grant number 03120321), the National Natural Science Foundation of China (grant number 31901690), the Scientific Research Fund Project of Department of Science and Technology of Sichuan Province (grant number 2018JY0149), and the Technology Innovation Program of Chinese Academy of Agricultural Sciences (grant number ASTIP). |