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Shang Ao, Luo Min, Gan Ren-You, Xu Xiao-Yu, Xia Yu, Guo Huan, Liu Yi, Li Hua-Bin. Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyusRehd.). ANTIOXIDANTS. DOI: 10.3390/antiox9080678. AUG 2020
来源:ANTIOXIDANTS
发布时间:2020-09-17
作者:甘人友、夏宇、郭欢、刘毅
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作者: Shang, A (Shang, Ao); Luo, M (Luo, Min); Gan, RY (Gan, Ren-You); Xu, XY (Xu, Xiao-Yu); Xia, Y (Xia, Yu); Guo, H (Guo, Huan); Liu, Y (Liu, Yi); Li, HB (Li, Hua-Bin) | ||||||||||
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影响因子 (2019 年): 5.014 | ||||||||||
摘要: In this study, the effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyusRehd.) were studied and the antioxidants in the extract were identified. The influences of ethanol concentration, solvent-to-sample ratio, microwave power, extraction temperature and extraction time on Trolox equivalent antioxidant capacity (TEAC) value, ferric reducing antioxidant power (FRAP) value and total phenolic content (TPC) were investigated by single-factor experiments. The response surface methodology (RSM) was used to study the interaction of three parameters which had significant influences on antioxidant capacity including ethanol concentration, solvent-to-sample ratio and extraction time. The optimal conditions for the extraction of antioxidants from sweet tea were found as follows-ethanol concentration of 58.43% (v/v), solvent-to-sample ratio of 35.39:1 mL/g, extraction time of 25.26 min, extraction temperature of 50 celcius and microwave power of 600 W. The FRAP, TEAC and TPC values of the extract under the optimal conditions were 381.29 +/- 4.42 mu M Fe(II)/g dry weight (DW), 613.11 +/- 9.32 mu M Trolox/g DW and 135.94 +/- 0.52 mg gallic acid equivalent (GAE)/g DW, respectively. In addition, the major antioxidant components in the extract were detected by high-performance liquid chromatography with diode array detection (HPLC-DAD), including phlorizin, phloretin and trilobatin. The crude extract could be used as food additives or developed into functional food for the prevention and management of oxidative stress-related diseases. | ||||||||||
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语言: English | ||||||||||
文献类型: Article | ||||||||||
作者关键词: sweet tea; Lithocarpus polystachyusRehd; antioxidant; microwave-assisted extraction; response surface methodology | ||||||||||
KeyWords Plus: RESPONSE-SURFACE METHODOLOGY; ENDOTHELIAL DYSFUNCTION; OPTIMIZATION; POLYPHENOLS; PHLORETIN; INGREDIENTS; PHLORHIZIN; MACERATION; REHD.; RSM | ||||||||||
地址: [Shang, Ao; Luo, Min; Xu, Xiao-Yu; Li, Hua-Bin] Sun Yat Sen Univ, Sch Publ Hlth, Dept Nutr, Guangdong Prov Key Lab Food Nutr & Hlth, Guangzhou 510080, Peoples R China. [Gan, Ren-You; Xia, Yu; Guo, Huan; Liu, Yi] Natl Agr Sci & Technol Ctr, Chengdu 610213, Peoples R China. [Gan, Ren-You; Xia, Yu; Guo, Huan; Liu, Yi] Chinese Acad Agr Sci, Inst Urban Agr, Res Ctr Plants & Human Hlth, Chengdu 610213, Peoples R China. |
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通讯作者地址: Li, HB (corresponding author),Sun Yat Sen Univ, Sch Publ Hlth, Dept Nutr, Guangdong Prov Key Lab Food Nutr & Hlth, Guangzhou 510080, Peoples R China. | ||||||||||
电子邮件地址: shangao@mail2.sysu.edu.cn; luom65@mail2.sysu.edu.cn; ganrenyou@caas.cn; xuxy53@mail2.sysu.edu.cn; xiayu01@caas.cn; ghscny@163.com; liuyi03@caas.cn; lihuabin@mail.sysu.edu.cn | ||||||||||
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Web of Science 类别: Biochemistry & Molecular Biology; Chemistry, Medicinal; Food Science & Technology | ||||||||||
研究方向: Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Food Science & Technology | ||||||||||
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The study was supported by the National Key R&D Program of China (No. 2018YFC1604405), the Local Financial Funds of National Agricultural Science & Technology Center (NASC), Chengdu, China (NASC2020KR02) and Central Public-interest Scientific Institution Basal Research Fund (No. Y2020XK05) and the Key Project of Guangdong Provincial Science and Technology Program (No. 2014B020205002). |
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